Product Overview
This in vitro qualitative diagnostic kit enables simultaneous detection and differentiation of Salmonella spp. and Shigella spp. nucleic acid in human stool specimens. Both are leading causes of bacterial gastroenteritis worldwide, and their clinical presentations overlap with each other and with other enteric pathogens — making simultaneous PCR the most efficient approach to etiological diagnosis. ### Key Features - **Dual-pathogen detection:** Salmonella and Shigella di
Key Features & Advantages
- - Dual - pathogen detection: - Salmonella and Shigella differentiated via two fluorescence channels. - - Target genes: - invA (Salmonella), ipaH (Shigella/EIEC). -
Clinical Applications
Clinical laboratories, hospitals, public health departments, and diagnostic testing centers for infectious disease detection.
Comparison & Competitive Advantages
Unlike conventional culture methods that require 48-72 hours for results, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. The molecular method offers superior analytical sensitivity, detecting low-copy-number targets that culture may miss.
Technical Specifications
Sample: types:** Stool, rectal swab, bacterial isolate. - **Analytical sensitivity:** ≤ 10 CFU/reaction equivalent. - **Turnaround time:** ~2 hours vs. 48–72 hours for culture. ### Clinical Applications - Acute bacterial gastroenteritis etiological diagnosis - Pediatric diarrhea — rapid rule-in/rule-out for empiric antibiotic decisions - Foodborne outbreak investigation and source confirmation - Pre-travel and post-travel screening in high-risk populations - Antimicrobial resistance stewardship (culture follow-up for confirmed cases) ### Comparative Advantage Stool culture for Salmonella and Shigella requires selective enrichment (selenite broth, GN broth), plating on XLD/HE/SS agar, and biochemical/serological confirmation — minimum 48 hours to a negative report. Real-time PCR reduces this to 2 hours with equivalent or superior sensitivity, enabling same-day clinical decisions and earlier outbreak notification. ### Comparative Advantages Unlike traditional bacterial culture methods that require 48-72 hours for isolation and biochemical identification, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. Compared to serological and culture-based approaches, the molecular method offers superior analytical sensitivity—detecting low-copy-number targets that culture may miss—while eliminating the ambiguity of cross-reactivity in conventional immunoassays. ### Quality & Compliance CE-IVD marked. ISO 13485 certified. WHO Global Foodborne Infections Network (GFN) compatible.
sensitivity: ** ≤ 10 CFU/reaction equivalent. - **Turnaround time:** ~2 hours vs. 48–72 hours for culture. ### Clinical Applications - Acute bacterial gastroenteritis etiological diagnosis - Pediatric diarrhea — rapid rule-in/rule-out for empiric antibiotic decisions - Foodborne outbreak investigation and source confirmation - Pre-travel and post-travel screening in high-risk populations - Antimicrobial resistance stewardship (culture follow-up for confirmed cases) ### Comparative Advantage Stool culture for Salmonella and Shigella requires selective enrichment (selenite broth, GN broth), plating on XLD/HE/SS agar, and biochemical/serological confirmation — minimum 48 hours to a negative report. Real-time PCR reduces this to 2 hours with equivalent or superior sensitivity, enabling same-day clinical decisions and earlier outbreak notification. ### Comparative Advantages Unlike traditional bacterial culture methods that require 48-72 hours for isolation and biochemical identification, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. Compared to serological and culture-based approaches, the molecular method offers superior analytical sensitivity—detecting low-copy-number targets that culture may miss—while eliminating the ambiguity of cross-reactivity in conventional immunoassays. ### Quality & Compliance CE-IVD marked. ISO 13485 certified. WHO Global Foodborne Infections Network (GFN) compatible.
Frequently Asked Questions
Q: What is the detection limit of this Salmonella & Shigella PCR kit?
A: The kit achieves a limit of detection (LOD) of approximately 5 copies per reaction, enabling sensitive identification of low-level infections. This molecular method is significantly more sensitive than conventional culture or antigen-based approaches.
Q: What sample types are compatible with this kit?
A: This kit is optimized for the appropriate clinical specimen type (e.g., stool, blood, swab). The integrated internal control monitors extraction efficiency and detects PCR inhibition, ensuring reliable results across different sample matrices.
Q: How long does the PCR test take?
A: The real-time PCR assay delivers results in approximately 2 hours, compared to 48-72 hours required for conventional culture-based methods. This enables same-day clinical decisions and faster outbreak response.
Q: What instruments is this kit compatible with?
A: The kit is compatible with mainstream real-time PCR instruments including Applied Biosystems 7500, QuantStudio 5, Roche LightCycler 480, Bio-Rad CFX96, QIAGEN Rotor-Gene Q, and Analytik Jena qTOWER3.
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