Norovirus GI and GII Real-Time PCR Kit

Product Overview

This in vitro qualitative diagnostic kit enables simultaneous detection and differentiation of Norovirus genogroup I (GI) and genogroup II (GII) nucleic acid in human stool specimens. Noroviruses are the most common cause of acute viral gastroenteritis worldwide, characterized by highly contagious transmission through fecal-oral routes, contaminated food/water, aerosolized vomitus and contact with infected surfaces. This kit provides rapid and reliable molecular diagnostic evidence to support early clinical identification, targeted patient management and effective outbreak control for norovirus infections in healthcare settings, communities and foodborne disease surveillance systems. The assay utilizes TaqMan probe-based real-time fluorescent PCR technology. Highly specific primers and dual-labeled hydrolysis probes are designed targeting the conserved ORF1 and ORF2 gene regions of Norovirus GI and GII. Norovirus GI is detected via the FAM fluorescence channel, while Norovirus GII is detected via the VIC fluorescence channel. A human RNase P housekeeping gene internal control is integrated into the reaction system and detected via the ROX fluorescence channel, which effectively identifies potential PCR inhibition, verifies sample nucleic acid integrity and confirms reagent performance for each individual test. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per test, enabling sensitive identification of low-level viral loads in stool samples. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of common enteric pathogens including Rotavirus, diarrheagenic Escherichia coli, Vibrio cholerae, Listeria monocytogenes, Vibrio parahaemolyticus, Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, nontyphoidal Salmonella, Shigella species and other clinically relevant microorganisms. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both intra-lot and inter-lot reproducibility. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC50201NW-25T) and 50 tests per kit (REF: JC50201NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making.

Key Features & Advantages

- oral routes, contaminated food/water, aerosolized vomitus and contact with infected surfaces. This kit provides rapid and reliable molecular diagnostic evidence to support early clinical identification, targeted patient management and effective outbreak control for norovirus infections in healthcare settings, communities and foodborne disease surveillance systems. The assay utilizes TaqMan probe - based real - time fluorescent PCR technology. Highly specific primers and dual - labeled hydrolysis probes are designed targeting the conserved ORF1 and ORF2 gene regions of Norovirus GI and GII. Norovirus GI is detected via the FAM fluorescence channel, while Norovirus GII is detected via the VIC fluorescence channel. A human RNase P housekeeping gene internal control is integrated into the reaction system and detected via the ROX fluorescence channel, which effectively identifies potential PCR inhibition, verifies sample nucleic acid integrity and confirms reagent performance for each individual test. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per test, enabling sensitive identification of low - level viral loads in stool samples. It demonstrates high analytical specificity with no cross - reactivity with a comprehensive panel of common enteric pathogens including Rotavirus, diarrheagenic Escherichia coli, Vibrio cholerae, Listeria monocytogenes, Vibrio parahaemolyticus, Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, nontyphoidal Salmonella, Shigella species and other clinically relevant microorganisms. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both intra - lot and inter - lot reproducibility. It is fully compatible with all mainstream real

Clinical Applications

Clinical laboratories, hospitals, public health departments, and diagnostic testing centers for infectious disease detection.

Comparison & Competitive Advantages

Unlike conventional culture methods that require 48-72 hours for results, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. The molecular method offers superior analytical sensitivity, detecting low-copy-number targets that culture may miss.

Technical Specifications

• sample: nucleic acid integrity and confirms reagent performance for each individual test. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per test, enabling sensitive identification of low-level viral loads in stool samples. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of common enteric pathogens including Rotavirus, diarrheagenic Escherichia coli, Vibrio cholerae, Listeria monocytogenes, Vibrio parahaemolyticus, Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, nontyphoidal Salmonella, Shigella species and other clinically relevant microorganisms. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both intra-lot and inter-lot reproducibility. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC50201NW-25T) and 50 tests per kit (REF: JC50201NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making.
• limit of detection: (LOD) of 5 copies per test, enabling sensitive identification of low-level viral loads in stool samples. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of common enteric pathogens including Rotavirus, diarrheagenic Escherichia coli, Vibrio cholerae, Listeria monocytogenes, Vibrio parahaemolyticus, Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, nontyphoidal Salmonella, Shigella species and other clinically relevant microorganisms. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both intra-lot and inter-lot reproducibility. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC50201NW-25T) and 50 tests per kit (REF: JC50201NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making.

Frequently Asked Questions

Q: What is the detection limit of this Norovirus GI and GII PCR kit?

A: The kit achieves a limit of detection (LOD) of approximately 5 copies per reaction, enabling sensitive identification of low-level infections. This molecular method is significantly more sensitive than conventional culture or antigen-based approaches.

Q: What sample types are compatible with this kit?

A: This kit is optimized for the appropriate clinical specimen type (e.g., stool, blood, swab). The integrated internal control monitors extraction efficiency and detects PCR inhibition, ensuring reliable results across different sample matrices.

Q: How long does the PCR test take?

A: The real-time PCR assay delivers results in approximately 2 hours, compared to 48-72 hours required for conventional culture-based methods. This enables same-day clinical decisions and faster outbreak response.

Q: What instruments is this kit compatible with?

A: The kit is compatible with mainstream real-time PCR instruments including Applied Biosystems 7500, QuantStudio 5, Roche LightCycler 480, Bio-Rad CFX96, QIAGEN Rotor-Gene Q, and Analytik Jena qTOWER3.