Zika Virus/Dengue Virus/Chikungunya Virus Real-Time PCR Kit

Product Overview

This in vitro qualitative diagnostic kit enables simultaneous detection and differentiation of Zika virus (ZIKV), Dengue virus (DENV) and Chikungunya virus (CHIKV) RNA in a single clinical specimen. It is validated for use with human serum, EDTA whole blood and cerebrospinal fluid (CSF) for all three pathogens, and additionally supports detection of Zika virus RNA in human urine and amniotic fluid. This single-tube multiplex assay streamlines diagnostic workflows, reduces sample consumption and accelerates differential diagnosis for patients presenting with febrile illness consistent with mosquito-borne viral infections. The assay utilizes TaqMan probe-based real-time fluorescent PCR technology. Highly specific primers and dual-labeled hydrolysis probes are designed targeting conserved genomic regions: the Polyprotein gene of Zika virus, the 3'UTR region of Dengue virus and the NSP1 gene of Chikungunya virus. Each target is detected via a distinct fluorescent channel: FAM for Dengue virus, VIC for Zika virus and ROX for Chikungunya virus. A human RNaseP gene internal control labeled with CY5 is integrated into the reaction system to monitor potential PCR inhibition, verify sample nucleic acid integrity and validate assay performance for each individual test. This kit delivers superior analytical performance with a limit of detection (LOD) of 5 copies per reaction for all three viruses, enabling sensitive identification of low-level viral loads. It demonstrates exceptional analytical specificity with no cross-reactivity with a broad panel of related pathogens including Yellow fever virus, West Nile virus, Japanese encephalitis virus, Hepatitis C virus, influenza viruses and other common infectious agents. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, testing days and operators. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC30302NW-25T) and 50 tests per kit (REF: JC30302NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making and appropriate infection control measures.

Key Features & Advantages

- tube multiplex assay streamlines diagnostic workflows, reduces sample consumption and accelerates differential diagnosis for patients presenting with febrile illness consistent with mosquito - borne viral infections. The assay utilizes TaqMan probe - based real - time fluorescent PCR technology. Highly specific primers and dual - labeled hydrolysis probes are designed targeting conserved genomic regions: the Polyprotein gene of Zika virus, the 3'UTR region of Dengue virus and the NSP1 gene of Chikungunya virus. Each target is detected via a distinct fluorescent channel: FAM for Dengue virus, VIC for Zika virus and ROX for Chikungunya virus. A human RNaseP gene internal control labeled with CY5 is integrated into the reaction system to monitor potential PCR inhibition, verify sample nucleic acid integrity and validate assay performance for each individual test. This kit delivers superior analytical performance with a limit of detection (LOD) of 5 copies per reaction for all three viruses, enabling sensitive identification of low - level viral loads. It demonstrates exceptional analytical specificity with no cross - reactivity with a broad panel of related pathogens including Yellow fever virus, West Nile virus, Japanese encephalitis virus, Hepatitis C virus, influenza viruses and other common infectious agents. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, testing days and operators. It is fully compatible with all mainstream real - time PCR instruments including Bioperfectus STC

Clinical Applications

Clinical laboratories, hospitals, public health departments, and diagnostic testing centers for infectious disease detection.

Comparison & Competitive Advantages

Unlike conventional culture methods that require 48-72 hours for results, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. The molecular method offers superior analytical sensitivity, detecting low-copy-number targets that culture may miss.

Technical Specifications

• sample: consumption and accelerates differential diagnosis for patients presenting with febrile illness consistent with mosquito-borne viral infections. The assay utilizes TaqMan probe-based real-time fluorescent PCR technology. Highly specific primers and dual-labeled hydrolysis probes are designed targeting conserved genomic regions: the Polyprotein gene of Zika virus, the 3'UTR region of Dengue virus and the NSP1 gene of Chikungunya virus. Each target is detected via a distinct fluorescent channel: FAM for Dengue virus, VIC for Zika virus and ROX for Chikungunya virus. A human RNaseP gene internal control labeled with CY5 is integrated into the reaction system to monitor potential PCR inhibition, verify sample nucleic acid integrity and validate assay performance for each individual test. This kit delivers superior analytical performance with a limit of detection (LOD) of 5 copies per reaction for all three viruses, enabling sensitive identification of low-level viral loads. It demonstrates exceptional analytical specificity with no cross-reactivity with a broad panel of related pathogens including Yellow fever virus, West Nile virus, Japanese encephalitis virus, Hepatitis C virus, influenza viruses and other common infectious agents. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, testing days and operators. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC30302NW-25T) and 50 tests per kit (REF: JC30302NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making and appropriate infection control measures.
• limit of detection: (LOD) of 5 copies per reaction for all three viruses, enabling sensitive identification of low-level viral loads. It demonstrates exceptional analytical specificity with no cross-reactivity with a broad panel of related pathogens including Yellow fever virus, West Nile virus, Japanese encephalitis virus, Hepatitis C virus, influenza viruses and other common infectious agents. The assay exhibits excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, testing days and operators. It is fully compatible with all mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two standard specifications: 25 tests per kit (REF: JC30302NW-25T) and 50 tests per kit (REF: JC30302NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing exceeding 5 times should be avoided to maintain reagent stability. This CE certified product is intended for professional in vitro diagnostic use only. Test results should be interpreted by trained healthcare professionals in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings to support accurate clinical decision-making and appropriate infection control measures.

Frequently Asked Questions

Q: What is the detection limit of this Zika Virus/Dengue Virus/Chikungunya Virus PCR kit?

A: The kit achieves a limit of detection (LOD) of approximately 5 copies per reaction, enabling sensitive identification of low-level infections. This molecular method is significantly more sensitive than conventional culture or antigen-based approaches.

Q: What sample types are compatible with this kit?

A: This kit is optimized for the appropriate clinical specimen type (e.g., stool, blood, swab). The integrated internal control monitors extraction efficiency and detects PCR inhibition, ensuring reliable results across different sample matrices.

Q: How long does the PCR test take?

A: The real-time PCR assay delivers results in approximately 2 hours, compared to 48-72 hours required for conventional culture-based methods. This enables same-day clinical decisions and faster outbreak response.

Q: What instruments is this kit compatible with?

A: The kit is compatible with mainstream real-time PCR instruments including Applied Biosystems 7500, QuantStudio 5, Roche LightCycler 480, Bio-Rad CFX96, QIAGEN Rotor-Gene Q, and Analytik Jena qTOWER3.