Yellow Fever Virus Real-Time PCR Kit

Product Overview

This in vitro qualitative diagnostic kit is designed for the specific detection of Yellow Fever Virus (YFV) nucleic acid in human clinical specimens including whole blood, serum, plasma and urine. It is intended for use on individuals who meet the clinical and/or epidemiological criteria for yellow fever, providing rapid and reliable molecular evidence to support early clinical diagnosis and timely infection control measures. The assay is based on the real-time fluorescent PCR technology with TaqMan probe method. Specific primers and dual-labeled hydrolysis probes are designed targeting the highly conserved 3'UTR region of the Yellow Fever Virus genome. During amplification, the 5'→3' exonuclease activity of Taq polymerase cleaves the probe bound to the template, separating the reporter dye from the quencher dye and generating measurable fluorescent signals. The kit incorporates an internal control (IC) targeting the human RNaseP gene detected via the VIC fluorescence channel, which effectively identifies potential PCR inhibition and verifies the integrity of reagents and sample processing, ensuring the validity of each test result. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per reaction, enabling sensitive detection of low viral loads. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of related pathogens including Zika virus, Chikungunya virus, Dengue virus (types 1-4), West Nile virus, Japanese encephalitis virus and other hemorrhagic fever viruses. The assay shows excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, operators and testing days. It is compatible with most mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two specifications: 25 tests per kit (REF: JC40107NW-25T) and 50 tests per kit (REF: JC40107NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing (more than 5 times) should be avoided to maintain reagent stability. This product is CE certified and intended for professional in vitro diagnostic use only. Test results should be interpreted in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings by trained healthcare professionals.

Key Features & Advantages

- time fluorescent PCR technology with TaqMan probe method. Specific primers and dual - labeled hydrolysis probes are designed targeting the highly conserved 3'UTR region of the Yellow Fever Virus genome. During amplification, the 5'→3' exonuclease activity of Taq polymerase cleaves the probe bound to the template, separating the reporter dye from the quencher dye and generating measurable fluorescent signals. The kit incorporates an internal control (IC) targeting the human RNaseP gene detected via the VIC fluorescence channel, which effectively identifies potential PCR inhibition and verifies the integrity of reagents and sample processing, ensuring the validity of each test result. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per reaction, enabling sensitive detection of low viral loads. It demonstrates high analytical specificity with no cross - reactivity with a comprehensive panel of related pathogens including Zika virus, Chikungunya virus, Dengue virus (types 1 - 4), West Nile virus, Japanese encephalitis virus and other hemorrhagic fever viruses. The assay shows excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, operators and testing days. It is compatible with most mainstream real - time PCR instruments including Bioperfectus STC - 96A, STC - 96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio - Rad CFX96™, QIAGEN Rotor

Clinical Applications

Clinical laboratories, hospitals, public health departments, and diagnostic testing centers for infectious disease detection.

Comparison & Competitive Advantages

Unlike conventional culture methods that require 48-72 hours for results, this real-time PCR kit delivers definitive pathogen detection in under 2 hours. The molecular method offers superior analytical sensitivity, detecting low-copy-number targets that culture may miss.

Technical Specifications

• sample: processing, ensuring the validity of each test result. This kit delivers exceptional analytical performance with a limit of detection (LOD) of 5 copies per reaction, enabling sensitive detection of low viral loads. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of related pathogens including Zika virus, Chikungunya virus, Dengue virus (types 1-4), West Nile virus, Japanese encephalitis virus and other hemorrhagic fever viruses. The assay shows excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, operators and testing days. It is compatible with most mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two specifications: 25 tests per kit (REF: JC40107NW-25T) and 50 tests per kit (REF: JC40107NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing (more than 5 times) should be avoided to maintain reagent stability. This product is CE certified and intended for professional in vitro diagnostic use only. Test results should be interpreted in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings by trained healthcare professionals.
• limit of detection: (LOD) of 5 copies per reaction, enabling sensitive detection of low viral loads. It demonstrates high analytical specificity with no cross-reactivity with a comprehensive panel of related pathogens including Zika virus, Chikungunya virus, Dengue virus (types 1-4), West Nile virus, Japanese encephalitis virus and other hemorrhagic fever viruses. The assay shows excellent precision with coefficient of variation (CV) values less than 5% for both repeatability and reproducibility across different batches, operators and testing days. It is compatible with most mainstream real-time PCR instruments including Bioperfectus STC-96A, STC-96A PLUS, Applied Biosystems 7500, QuantStudio™ 5, Roche LightCycler® 480, Bio-Rad CFX96™, QIAGEN Rotor-Gene Q and Analytik Jena qTOWER3. The kit is available in two specifications: 25 tests per kit (REF: JC40107NW-25T) and 50 tests per kit (REF: JC40107NW-50T). All components should be stored at -20±5°C protected from light, and repeated freezing and thawing (more than 5 times) should be avoided to maintain reagent stability. This product is CE certified and intended for professional in vitro diagnostic use only. Test results should be interpreted in conjunction with patient clinical history, signs and symptoms, and other diagnostic findings by trained healthcare professionals.

Frequently Asked Questions

Q: What is the detection limit of this Yellow Fever Virus PCR kit?

A: The kit achieves a limit of detection (LOD) of approximately 5 copies per reaction, enabling sensitive identification of low-level infections. This molecular method is significantly more sensitive than conventional culture or antigen-based approaches.

Q: What sample types are compatible with this kit?

A: This kit is optimized for the appropriate clinical specimen type (e.g., stool, blood, swab). The integrated internal control monitors extraction efficiency and detects PCR inhibition, ensuring reliable results across different sample matrices.

Q: How long does the PCR test take?

A: The real-time PCR assay delivers results in approximately 2 hours, compared to 48-72 hours required for conventional culture-based methods. This enables same-day clinical decisions and faster outbreak response.

Q: What instruments is this kit compatible with?

A: The kit is compatible with mainstream real-time PCR instruments including Applied Biosystems 7500, QuantStudio 5, Roche LightCycler 480, Bio-Rad CFX96, QIAGEN Rotor-Gene Q, and Analytik Jena qTOWER3.